Sofia Nicastro ’25 ChE Shares Her Perspective on the World of Synthetic Biology

Every student knows the dreaded feeling of course registration—the uncertainty surrounding figuring out what courses are required, determining what electives to take, and seeing what times classes are offered. When I was preparing for registration last spring, a new Chemical Engineering elective caught my eye: Synthetic Biology, taught by Visiting Assistant Teaching Professor Justin Vento, PhD. I decided to enroll.

This course was special in that it had both lecture and laboratory components, was project-focused, and even included a podcast. During the lectures, we learned about synthetic biology principles, which we then applied during lab time to a semester-long project.

Our goal was to genetically engineer yeast to produce beta-carotene, a vitamin A precursor and one of the chemicals that makes foods like carrots orange. This is a more sustainable method of producing important compounds than chemically synthesizing them.

We investigated multiple strategies for yeast engineering, including using CRISPR (or clustered regularly interspaced short palindromic repeats) to modify the yeast’s DNA to produce beta-carotene inside the cell. I am proud to report that our efforts were successful: The yeast cells turned orange!

This class took place in the Gary A. Gabriele Classroom, a brand-new lab and lecture space in Drosdick Hall named for the dean of the College of Engineering from 2006 to 2018. This unique space can facilitate both lectures and labs in the same room, allowing for a seamless transition between experiments and notetaking. This enabled us to make the best use of our class time: We could perform experiments on both class days and switch to lecture while in in the experiments’ waiting periods.

Synthetic biology is something I previously did not know much about, but I now have a much greater understanding of its use and the types of careers in this field. Since this class had a lab portion, I learned valuable skills that can only be acquired through lab work.

Outside of the classroom, I am co-vice president for Best Buddies, co-chair for the Society of Women Engineers’ Service and Outreach Committee, and president of Villanova’s chapter of the American Institute of Chemical Engineers, my favorite of the roles. I have served on the AIChE executive board for the past three years—one of my most rewarding college experiences. One hugely beneficial part of AIChE was learning from the upperclassmen. Now that I am a senior, I love helping the underclassmen with all things related to our major and Villanova in general.

After graduation, I will pursue a career in the pharmaceutical and life sciences industry, and I hope to use my knowledge to make products that help people live better lives.

Sofia Nicastro is a senior from Deptford, N.J., majoring in Chemical Engineering and minoring in Math, Business, and Biochemical Engineering.