Use of DNA plasmids in future gene therapy or DNA vaccine products is currently cost-prohibitive, mainly due to the expensive chromatography steps necessary for final product purification. The goal of this project is to explore a novel and less expensive approach to traditional chromatography. Small DNA sequences have been designed for insertion into the plasmids, that the potential to adsorb to inexpensive powders.
Expanded bed adsorption is attractive in the recovery of bio-pharmaceuticals, because this single step can achieve purification without the need/cost of a cell removal (i.e. filtration) step. Experiments will be conducted to determine conditions that minimize interaction between the cells and resin, while maximizing the selective adsorption of the protein bio-product on the resin. This is a collaborative project with researchers at Glaxo-SmithKline.
Fluent is a CFD software that is windows based, and computes/displays flow in 3D geometries. The results of our CFD model of oxygen transfer in a bioreactor are being compared to actual experimental data from our laboratory. The CFD model can then be used to determine the optimum bioreactor configuration (i.e. sparger location etc…) for given operating conditions (RPM, type cell line..).