Microscope Tutorials

Leica TCS SP8 Four Channel Spectral Confocal Microscope

Confocal microscopy is an optical imaging technique where by the optical resolution is increased by the addition of a spatial pinhole placed at the confocal plane of the lens to eliminate the out-of-focus light from above and below the point of focus in an object.  Our Leica SP8 inverted confocal microscope (acquired in May, 2016) provides the ability to acquire high-resolution, optically sectioned images from within a thick sample (in either fixed or living cells and tissue) or to reduce background fluorescence from thin cultures.  Other features of the SP8 include: high sensitivity point confocal, high-sensitivity GaAsP HyD detectors with photon counting, spectrally tunable emission bands, a resonant scanner for low photo-toxicity, 3D z-stacks, time lapse imaging, stitching, and multi-position time lapse imaging.

confocal microscope

Principles of Confocal Microscopy

Leica SP8 Confocal Microscope Tutorial

Leica LAS X 3D Analysis Tutorial