Faculty Experts - Jens O.M. Karlsson, Ph.D.

Jens O.M. Karlsson, Ph.D.

Jens O.M. Karlsson, Ph.D.
Associate Professor
Department of Mechanical Engineering
Cellular and Molecular Bioengineering Research Group

Area of Expertise:

  • Cryopreservation of Cells and Tissue Engineered Devices
  • Cryosurgery
  • Hyperthermic processes, including burn injury, thermal ablation, heat shock response, fever.

Summary

Dr. Karlsson is a leading expert in the biothermal sciences. These include cryobiology (cooling) and hyperthermic processes (heating). Cryobiology investigates cryopreservation and cryosurgery. Cryopreservation is used in cell banking for many areas of biotechnology. It is also used for cell therapy (stem cells), in vitro fertilization, blood cell banking. Cryosurgery can be used to destroy certain cancerous cells. Hyperthermic processes include the treatment of burn injuries and fever. They also cover thermal ablation (removal), used in the treatment of cancers and in transdermal drug delivery.

Education

  • Ph.D., Massachusetts Institute of Technology
  • S.M., Massachusetts Institute of Technology
  • S.B., Massachusetts Institute of Technology

Selected Publications

  • Karlsson, J.O.M., A.I. Younis, A.W.S. Chan, K.G. Gould, A. Eroglu, "Permeability of the rhesus monkey oocyte membrane to water and common cryoprotectants," Molecular Reproduction and Development 76:321-333 2009.
  • Stott, S.L., J.O.M. Karlsson, "Visualization of intracellular ice formation using high-speed video cryomicroscopy," Cyberbiology 58: 84-95, 2009.
  • Irimia, D., J.O.M. Karlsson, "Kinetics of intracellular ice formation in one-dimensional arrays of interacting biological cells," Biophysical Journal 88:647-660, 2005.
  • Stott, S.L., Irimia, D., J.O.M. Karlsson, "Parametric analysis of intracellular ice propagation during cryosurgery, simulated using Monte Carlo techniques," Technology in Cancer Research and Treatment 3: 113-124, 2004. [Science Applications International Corporation Student Paper Award]
  • Irimia, D., J.O.M. Karlsson, "Development of a cell patterning technique using polyethylene glycol disilane," Biomedical Microdevices 5: 185-194, 2003.
  • Karlsson, J.O.M., "A theoretical model of intracellular devitrification," Cryobiology 42:154-169, 2001.
  • Karlsson, J.O.M., M.L. Yarmush, M. Toner, "Interaction between heat shock and interleukin 6 simulation in the acute-phase response of human hepatoma (HepG2) cells," Hepatology 28: 994-1004, 1998.
  • Karlsson, J.O.M., M. Toner, "Long term storage of tissues by cryopreservation: Critical issues," Biomaterials 17:243-256, 1996.

Selected Professional Experience

  • Karlsson, J.O.M., A.I. Younis, A.W.S. Chan, K.G. Gould, A. Eroglu. “Permeability of the rhesus monkey oocyte membrane to water and common cryoprotectants.” Molecular Reproduction and Development, 76:321-333 (2009).
  • Stott, S.L., J.O.M. Karlsson. Visualization of intracellular
  • Irimia, D., J.O.M. Karlsson. “Kinetics of intracellular ice formation in one-dimensional arrays of interacting biological cells.” Biophysical Journal 88:647-660, 2005.
  • Stott, S.L., Irimia, D., J.O.M. Karlsson. Parametric analysis of intracellular ice propagation during cryosurgery, simulated using Monte Carlo techniques. Technology in Cancer Research and Treatment 3: 113-124, 2004 [Science Applications International Corporation Student Paper Award]
  • Irimia, D., J.O.M. Karlsson. Development of a cell patterning technique using polyethylene glycol disilane. Biomedical Microdevices 5: 185-194, 2003.
  • Karlsson, J.O.M., “A theoretical model of intracellular devitrification.” Cryobiology 42:154-169, 2001.
  • Karlsson, J.O.M., M.L. Yarmush, M. Toner. Interaction between heat shock and interleukin 6 simulation in the acute-phase response of human hepatoma (HepG2) cells. Hepatology 28: 994-1004, 1998.
  • Karlsson, J.O.M., M. Toner. “Long term storage of tissues by cryopreservation: Critical issues.” Biomaterials 17:243-256, 1996.

Selected Research

  • Sugars As Novel Cryoprotectants for Primate Oocytes, Medical College of Georgia (NIH R01 Subcontract); Principal Investigator on Subcontract (Prime PI: A. Eroglu, Medical College of Georgia), 2007-2009.
  • Kinetics of Intracellular Ice Formation During Cryopreservation of Tissue Engineered Constructs, National Science Foundation (BES-Biotechnology), Principal Investigator, 2006-2009.
  • Optimized Thawing of Cryopreserved Skin Cells, Whitaker Foundation (Biomedical Engineering Research Grant), Principal Investigator, 1998-2001.
  • Mechanism of Thermal Microporation of Skin Using a Hot-Filament Technology for Transdermal Drug Delivery, Altea Therapeutics Corporation, and Georgia Research Alliance (Innovation Grant), Principal Investigator (Co-Investigator: Z. Zhang, Georgia Tech), 2003-2006.
  • CAREER: Rational Design of Cryopreservation Techniques for Cells and Tissues, National Science Foundation (Faculty Early Career Development Award), Principal Investigator, 1999-2004.

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