Environmental Engineering Laboratories

Environmental Engineering Laboratories

Director: Metin Duran, PhD, Professor, Department of Civil and Environmental Engineering

Focus: Engaged in research and education in the applied microbiology and aquatic chemistry area as it relates engineering solutions to public health and environmental protection.

Research Highlights:

  • Public health microbiology (microbial water quality and microbial source tracking) and biological processes for waste management (anaerobic processes and biofuel from waste).
  • Remediation of recalcitrant contaminants and pathogens in drinking water and wastewater by the development of novel electrochemical and catalytic technologies.
  • Quantifying and removing trace contaminants (e.g., disinfection byproducts, hormones, pharmaceuticals, and personal care products) in natural waters.

Facility Highlights:

The Environmental Engineering Laboratory facilities include:

 

1.  Core Genomics Laboratory (CEER 106 and 108), featuring:

  • Applied Biosystems 7300 RT-PCR system
  • Shimadzu BioSpec-mini UV-Vis
  • Spectrophotometer optimized  for rapid quantification of RNA, DNA, and proteins
  • NanoDrop spectrophotometer for quantification of RNA and DNA in microliter sample volumes
  • Microscopy suite with a Leica DM2000 upright microscope and a Leica DMI3000 inverted microscope, equipped with epifluorescent illumination and a Q-Imaging Retiga-SRV cooled CCD camera for imaging of cells and molecular probes
  • Eppendorf 5417R refrigerated high-speed mini centrifuge
  • Milli-Q Biocell water purification system that supplies RNAase free water
  • Beckman-Coulter Vi-CELLTM cell viability analyzer
  • Molecular Dynamics Gemini EM fluorescence plate reader
  • Shimadzu HPLC with UV-Vis detector
  • Sonicator for cell lysis
  • Ultra-low freezer (-85oC); a basic freezer (-20oC); and a crushed ice maker

2. The Environmental Microbiology and Biotechnology Laboratory (CEER 306), featuring:

  • A 110-ft2 environmental room maintained at 35 ±2oC for research related to anaerobic processes
  • Analytical infrastructure incorporating several molecular biology tools, including PCR, FAME, and gel electrophoresis
  • 10-ft anaerobic glove box for strict anaerobe culturing

 

3.  The Analytical Laboratory (CEER Room 311), featuring:

  • Hewlett Packard gas chromatograph with mass spectrophometer detector (GC/MS) with ion and electron ionization capabilities
  • Two Hewlett Packard 6890 gas chromatographs with flame ionization, thermal conductivity and electron capture detectors
  • Shimadzu HPLC with electrochemical and UV detectors
  • Dionex DX-600 IC with  electrochemical and photodiode array detectors
  • Pectronic Genesys 5 variable wavelength scanning UV/Vis spectrophotometer