Biochemical Engineering and BioProcessing Laboratory

Biochemical Engineering and BioProcessing Laboratory

Director: William Kelly, PhD, Associate Professor, Department of Chemical Engineering

Focus: Research and education in biochemical engineering primarily as it relates to the optimization, modeling and scale-up of biopharmaceutical processes.

Research Highlights:

  • Exploration of novel and less expensive approaches to traditional chromatography, which is required to purify DNA plasmids for use in gene therapy or DNA vaccine products.
  • Study of the degree to which bioreactor, centrifuge and filter design and operating conditions damage Chinese Hamster Ovary cells, which are often used for the production of monoclonal antibodies.
  • Use of the Computational Fluid Dynamic model to determine the optimum bioreactor configuration for given operating conditions.

Facility Highlights:

The Biochemical Engineering and BioProcessing lab consists of four adjacent but isolated suites:

  • Cell culture suite with:
    • Laminar flow hood
    • Two 20-liter steam-in-place Bioflow IV bioreactors
    • CO2incubator/cabinet
    • Two-liter glass fermentor
  • Fermentation suite with:
    • Laminar flow hood
    • Shaker cabinet
    • One 20-liter steam-in-place Bioflow IV bioreactor
    • Two two-liter glass fermenters
    • Incubator  
  • Isolation room with:
    • State-of-the-art Gaulin homogenizer
    • Continuous flow CARR basket-type centrifuge
    • Millipore filter
  • Bio-analytical laboratory with:
    • Benchtop centrifuges
    • Microscopes
    • Spectrometer
    • Gel electrophoresis equipment