Instrumentation

The research and teaching laboratories in the Biochemistry program utilize instrumentation and facilities from both the Department of Chemistry and Biology. 

Major instrumentation located in the Department of Biology includes

  • scanning and transmission electron microscopes
  • a laser capture microdissection microscope
  • fluorescence microscopes
  • phase contrast microscopes
  • numerous brightfield and dissecting microscopes.
  • flow cytometers (2)
  • thermal cyclers for quantitative PCR (2)
  • several gradient PCR cyclers
  • several standard thermal cyclers
  • plate readers with fluorescence/luminescence capacity 
  • X-ray film processor
  • gel documentation systems, including super cooled cameras
  • several UV-VIS spectrophotometers including two Nanodrop spectrophotometers
  • a 96 capillary automated DNA sequencer
  • an ultracentrifuge, several superspeed centrifuges, and clinical centrifuges
  • laminar flow hoods (11) with nearby water jacketed CO2 incubators
  • a variety of standard incubators, both  shaking and stationary
  • extensive liquid nitrogen and ultra-low temperature storage capacity. 
  • walk-in cold rooms (2)
  • autoclaves (3)
  • dishwashers and ovens available for lab preparation. 
  • A vivarium is located in Mendel Science Center

Major instrumentation in The Department of Chemistry includes

  • BioRad Benchmark Plus UV-Vis plate reader
  • BioRad iMark UV-Vis plate reader
  • UVP EC3 imaging system
  • Techne TC3000G thermal cycler
  • BioRad T100 thermal cyclers (2)
  • New Brunswick Excella E25 incubator/shaker
  • New Brunswick C25KC incubator/shaker
  • Sorvall RCB5 Plus refrigerated high speed centrifuge and rotors
  • Sorvall RC5C refrigerated high speed centrifuge and rotors
  • Kendro-Sorvall ultra-microcentrifuge and rotors
  • Multiple Nanopure water purification systems
  • New Brunswick Ultra low temperature Freezer
  • U725 Innova So-Low Environmental ultra low temperature freezer
  • Liquid nitrogen cell storage system
  • Branson sonifiers (2): 450W and 250W with microtip and cuphorn
  • Laminar flow hoods (2)
  • water-jacketed CO2 incubators (3)
  • EVOS XL Core inverted light microscope (bright phase and phase contrast) with digital screen and camera
  • Motic AE2000 inverted light microscope
  • Thermo Scientific Biomate 6 UV-Vis spectrophotometer
  • BTX electroporation system
  • Hybaid hybridization oven
  • UVP CC-1000 UV crosslinker
  • SNAP i.d. Protein Detection System
  • Bio-Rad BioLogic DuoFlow 10 FPLC
  • Avestin EmulsiFlex-C5 high-pressure homogenizer
  • Bio-Rad Personal Molecular Imager (phosphorimager)
  • Perkin Elmer AAnalyst 100 and 300 Atomic absorption spectrophotometers
  • Fluourimeters (3)
  • Perkin Elmer Spectrum One FTIR (3)
  • Perkim Elmer FX FTIR
  • Varian Mercury 300 and XL-300 NMRs
  • Perkin Elmer Lambda 35 UV-Vis spectrophotometer
  • Bruker X-Ray Diffractometer
  • Perkin Elmer Polarimeter
  • Bruker Tracer III-V SD Handheld XRF
  • Jarell Ash ICP Atomic Emission Spectrophotometer
  • Multilpe chemiluminescence analyzers (nitrogen and sulfur)
  • Shimadzu QP-2010 Plus GC/MS Varian 3400/Finnigan MAT ion-trap GC/MS
  • Varian 3900/2100T ion-trap GC/MS
  • Hewlett Packard 5890 GC/FID (3)
  • Shimadzu GC/FID
  • Thermo Trace GC/ECD  Waters 600/480 LC HPLC
  • Varian Prostar Thermo and HP 1050  HPLCs
  • Lumex International Capel-150 Capillary Electrophoresis
  • Shimadzo Prominence HPLC (3)
  • Applied Biosystems 3200 Q-TRAP MS/MS  and API 2000 MS/MS
  • Thermodyne Tube Furnace
  • Differential scanning calorimeter 
  • thin film spinner
  • glove boxes (3)
  • solvent purification system
  • Dionex ASE200 Accelerated Solvent extractor

Villanova Biochemistry at a Glance

Degrees Offered: BS

Core Faculty: 14

Undergraduate Majors: 114

Equipment: Complete array of modern instrumentation.

Building:  Mendel Science Center

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